Delphine Guillebault

Fonction et rattachement

Actuellement dirigeante de la start-up Microbia Environnement hébergée à l'Observatoire Océanologique de Banyuls sur mer

www.microbiaenvironnement.com

Carrière

10/2010- Present Research assistant in the Microbial biodiversity and biotechnology research team, Université Pierre et Marie Curie–CNRS, UMR 7621. Supervisor : Pr. Philippe Lebaron

Investigation of cyanobacteria diversity in thermal environments

09/2007- 08/2009 Teaching Assistant in Environmental Microbiology, Université Pierre et Marie Curie

Design of molecular tools combined to Flow Cytometry to assess genetic diversity of marine microbes

Analysis of cytometric bacterial groups using the newly designed tools

04-08/2007 Research Assistant in Microbial biodiversity and biotechnology group, Université Pierre et Marie Curie–CNRS, UMR 7621. Supervisor : Pr. Philippe Lebaron

Design of molecular tools combined to Flow Cytometry to assess genetic diversity of marine microbes

09/2006-02/2007 Research Assistant (CNRS) in the Comparative genomics of phytoplankton research team Université Pierre et Marie Curie–CNRS, UMR7628. Supervisor: Dr. Hervé Moreau’

Investigation of the cytoplasmic pathway of starch biosynthesis in the dinoflagellates Crypthecodinium cohnii, (red versus green lineage)

11/2004- 08/2006 Maternity and Parental leave

11/2001-10/2004 Research Assistant in Dr. Sue Cotterill’s group, Basic Medical Sciences Department St Georges Hospital Medical School, London, United Kingdom

Functional study of the original drosophila protein Df31

Protein biochemistry, Genetics and molecular biology tools

10/1998-10/2001 PhD student in Cellular and Molecular Biology in Dr. Hervé Moreau’s group Université Pierre et Marie Curie–CNRS, UMR7628

06-08/1996 and 01-06/1997 Technician in Dr. Bruce Roe’s laboratory, University of Oklahoma, USA

Participation to the Human Genome Sequencing Consortium

Sequencing training on the Human Chromosome 22

TEACHING EXPERIENCE

Teaching assistant in environmental microbiology, molecular tools applied to microbial diversity studies and bioinformatics (use of particular software) (BA, MA and PhD students)

Supervision of students during the European Intensive Course Program (ERASMUS) from the 10^th to the 24^th of July 2000 titled “Marine Cell Biology: Marine models for the study of cell cycle and development.”

Oral Communications

GUILLEBAULT D. , CREVEL G, COTTERILL S (2003) “DF31, a protein involved in the chromatin architecture”, Saint Georges Hospital Medical School, Londres, Royaume-uni.

GUILLEBAULT D. , DERELLE E., BHAUD Y., GERAUD M.-L., ALBERT M., SOYER-GOBILLARD M.-O., MOREAU H. (2001).Thesis presentation to the members of the jury of the prize Daniel Jouvance.

GUILLEBAULT D. , DERELLE E., BHAUD Y., GERAUD M.-L., ALBERT M., SOYER-GOBILLARD M.-O., MOREAU H. (2001). “Chromatin and transcription in the heterotrophic dinoflagellate Crypthecodinium cohnii”, Saint Georges Hospital Medical School, Londres, Royaume-uni.

International Meetings

4^th International conference on analysis of microbial cells at the single cell level, Bad Schandau, Germany, May 22^nd-25^th 2008.

Poster: Guillebault D. , Catala P. and Lebaron P. « Molecular analysis of HNA and LNA bacterioplankton using flow cytometer and polymerase chain reaction.»

9^th International Conference on Harmful Algal Blooms, Hobart, Tasmania, Australia, 7-11 Mars 2000.

Poster : GUILLEBAULT D. , DERELLE E., BHAUD Y., GERAUD M.-L., ALBERT M., SOYER-GOBILLARD M.-O., MOREAU H. (2000). Nuclear proteins potentially involved in the control of the transcription in the heterotrophic dinoflagellate Crypthecodinium cohnii.

9th International Genome Sequencing and Analysis Conference. Hilton Head, South Carolina, USA. September 13-16, 1997.

Poster: Hua-Qin Pan, cRABTREE J., MALAJ’ E., Guillebault D. , DUMANSKI J. and ROE B. A. “Large Scale Sequencing of Three Meningioma Deletion Regions on Chromosome 22”. Microbial & Comparative Genomics. 1997, 2(3): 198-228.

SCIENTIFIC COLLABORATIONs

Dr. Laszlo Tora , CNRS/INSERM/Université Louis Pasteur., ILLKIRCH, France (2001)

Pr. Steven Ball , UMR 8576, Université des Sciences et Technologies de Lille, France (2006-2007)

Dr Eric Viscogliosi , Inserm U547, Institut Pasteur de Lille, France (2007)

Publications

2010

GUILLEBAULT D. , LaGhDass M., Catala P., obernosterer I. and Lebaron P. (2010) “An improved method of bacterial cell capture after flow cytometry cell sorting” Applied Environmental Microbiology, Published ahead of print on 3 September 2010, doi:10.1128/AEM.00621-10, November 2010, p. 7352-7355, Vol. 76, No. 21 NB: IF 2009 = 3.686

2008

Deschamps P., Guillebault D. , Devassine J., Dauvillee D., Haebel S., Steup M., Buleon A., Putaux J. L., Slomianny M. C., Colleoni C., Devin A., Plancke C., Tomavo S., Derelle E., Moreau H. and Ball S. (2008) “The heterotrophic dinoflagellate Crypthecodinium cohnii defines a model genetic system to investigate cytoplasmic starch synthesis.” Eukaryotic Cell 7: 872-880. IF 2008: 3.83

DUFERNEZ F., DERELLE E., NOEL C., SANCIU G., MANTINI C., DIVE D., SOYER-GOBILLARD M.O., CAPRON M., PIERCE R.J., WINTJENS R., GUILLEBAULT D. , VISCOGLIOSI E. (2008). “Molecular Characterization of Iron-Containing Superoxide Dismutases in the Heterotrophic Dinoflagellate Crypthecodinium Cohnii.” Protist, 159: 223-238. IF 2008: 3.262

2007

GUILLEBAULT D. , COTTERILL S. (2007). The Drosophila Df31 protein interacts with histone H3 tails and promotes chromatin bridging in vitro. J Mol Biol., 373(4):903-12. IF 2008: 4.146

2002

GUILLEBAULT D. , SASORITH S., DERELLE E., WURTZ J.M., LOZANO J.C., BINGHAM S., TORA L., MOREAU H. (2002). A new class of transcription initiation factor, intermediate between TBPs and TLFs is present in the marine unicellular organism: The dinoflagellate Crypthecodinium cohnii. J. Biol. Chem., 277(43): 40881-40886. IF 2008: 5.52

Soyer-Gobillard, M.O., Besseau, L., Géraud, M.L., Guillebault D. , Albert, M., Perret, E. (2002). Cytoskeleton and mitosis in the dinoflagellate Crypthecodinium cohnii: immunolocalization of P72, an HSP70-related protein. European Journal of Protistology, 38 (2), 155-170. IF 2008: 1

2001

GUILLEBAULT D. , DERELLE E., BHAUD Y., MOREAU H. (2001). Role of nuclear WW domains and prolin-rich proteins in Dinoflagellates transcription. Protist, 152: 127-138. IF 2008: 3.923

2000

BHAUD Y., GUILLEBAULT D. , LENNON J.-F., DEFACQUE H., SOYER-GOBILLARD M.-O., MOREAU H. (2000). Morphology and behaviour of dinoflagellate chromosomes during the cell cycle and mitosis. J. Cell Sci., 113: 1231-1239. IF 2008: 6.247

Prix et distinctions

May 2008 Workshop Grant for the 4th International Conference on Analysis of Microbial Cells at the Single-Cell Level, Bad Schandau, Dresden, Germany: 1 300 Euros

10/1998- 10/2001 Scholarship for PhD research work founded by the Ministère de l’Education Nationale de la Recherche et Techniques (MENRT),

2001 Winner of Daniel Jouvance Award for research work on marine phytoplankton: 1 500 Euros

7-11 March 2000 Workshop Grant for the 9^th International Conference on Harmful Algal Blooms. (Hobart, Tasmania, Australia,): 1 500 Euros

Etablissements fréquentés

Diplômes français et étrangers

10/1998- 10/2001 Ph.D. (Hons), Cellular and Molecular Biology, grade A (excellent), University of Montpellier II

Title: Chromatin and transcription in dinoflagellates: identification and characterization of nuclear proteins involved in transcription regulation.

10/1997-09/1998 M.A., Molecular Physiology and Genetics, University Blaise Pascal, Clermont Ferrand

Title: Characterization of dinoflagellates nuclear proteins

01/1997-06/1997 Certificate of completion for coursework in molecular biology and biochemistry, MS grade A (excellent), Spring Academic Term, The University of Oklahoma, Norman Campus

10/1995-05/1996 B.A, Cellular Biology and Physiology, University Blaise Pascal, Clermont Ferrand

06/1996-08/1996 Certificate of completion for Biology Research, Summer Academic Term, The University of Oklahoma, Norman Campus